Bio Imaging Facility
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| Description of the Facility |
 | Bio-Imaging
The Bio-Imaging core facility currently provides optical and video microscopy services. The facility is currently equipped for bright field, phase contrast, modulation contrast and epi fluorescence microscopy. The facility has two separate image analysis stations. A Nikon Optiphot microscope is equipped with a color video system and Compix imaging software, and a Nikon Eclipse TE200 inverted scope. This inverted scope in specially configured to perform calcium ratio imaging is equipped with Molecular Devices Meta Fluor software The recent acquisition of a Phosphor Imager and a Typhoon 9410 scanner, along with the purchase of a confocal microscope, allows for the quantitation and localization of sub cellular fluorescent and radioactive molecules. In addition the purchase of a Densitometer allows for the analysis of 1 and 2 D gels, Southern, northern and western blots and RFPL gels. The facility is also equipped with color laser printer and slide recorder for the production of publication quality graphics. and presentation materials |
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| Instruments |
 | Compix Color Image Analysis System
The Compix Imaging system consists of a Nikon Optiphot 2 upright microscope, and Nikon DXM 1200F high resolution (3,840 x 3,072 pixels) color video camera. The microscope is equipped for fluorescence, and has phase contrast and Nomarski optics. The system is interfaced to a 1.4Ghz PC which utilizes Compix Simple PCI image analysis software. The system also has Adobe Photoshop install for image acquisition and manipulation
Rate: The facility charges $5:00 per hour to use this system
To book the facility send email to williams@genectr.hunter.cuny.edu |
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 | Gemini EM microplate spectrofluorometer
The Molecular Devices SpectraMax Gemini EM microplate spectrofluorometer features top and bottom reading optics, dual monochromators, wavelength scanning, well scanning, auto PMT gain and is driven by Softmax Pro software on a Windows-based controller |
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 | Molecular Dynamics Typhoon 9410
Typhoon™ is a highly sensitive variable-mode gel imager. The Typhoon 9410 unites the ability to detect an extensive variety of fluors, with proven storage phosphor autoradiography technology and direct imaging of chemiluminescence. The typhoon can also be used to analyze micro-arrays. |
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 | PerkinElmer UltraVIEW ERS Spinning Disc Microscope
UltraVIEW ERS microscope is a spinning disk confocal microscope equipped with six laser lines, which allow visualization of GFP, RFP, CY5, DAPI, CFP and YFP, etc. The UltraVIEW ERS system allows for high-speed, multiple-probe, time-lapse experiments; fully automated image capture; time-lapse morphometric measurements; fast sequential image capture of multiple probes; and fast z-stack acquisition. UltraVIEW ERS Imaging Suite software and Volocity are used for fast image display, image acquisition and comprehensive image analysis. The system is supported by a Nikon Eclipse TE2000 inverted fluorescence microscope with environmental incubator which is ideal for live cell applications. |
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 | JEM-100C/CX Transmission Electron Microscope
JEOL JEM-100CX II transmission electron microscope is an advanced high performance electron microscope with stable and excellent imaging capabilities at low to high magnifications (90X to 800, 000X). A 10M-pixel HAMAMASTU C4742-95 digital camera is integrated to the system for high-resolution image acquisition. |
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 | Desktop Publishing and Presentation Tools
The Imaging Facility desktop publishing station has a Adobe Photoshop and PageMaker and Microsoft PowerPoint for creating high quality graphics. A Polaroid Pro Palette 8000 slide maker, and HP Color LaserJet 3800. There is a per page charge for the printers, the other facilities are free. |
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 | Leica Confocal Microscope TCS SP2
The TCS SP2 Laser Scanning Spectral Confocal Microscope can do measurements of transmitted light, fluorescence and laser scanning fluorescence imaging. It has a z-stage with rotation capabilities, with z-resolution of 40 nm. The system has high quality X10, X40, X63 and X100 objectives, and 6 excitation laser lines at wavelenghts of 458nm - 638 nm. Cost to use this instrument is $15/hr |
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 | PowerWave HT Microplate Spectrophotometer
PowerWave HT is a multi-channel reader for maximum speed in both 96- and 384-well plate formats. The PowerWave HT provides flexibility for multiple applications, including endpoint, kinetic and spectral scanning mode. Powerful Gen5™ PC-based software is used for system control and data analysis.
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 | Calcium Ratio & Micro Injection Inverted Scope
The calcium ratio imaging system consists of: a Nikon Eclipse TE 200 inverted epifluorescence microscope, sutter lambda 10-2 optical filter system, and Molecular Devices imaging software Metafluor. The system also is equiped with a Narishige micromanipulator system |
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| Rules of operations |
Guidelines For using The Facility
A. The facility is open for use by members of the Gene Center, other CUNY departments, and outside parties with the prior arrangement of the staff of the Bio-Imaging facility
B. Your use of the facility will be recorded. For the optical microscopes and the Gel and Blot scanners you must obtain a "Gene Center" computer account. This is required to log on to the computers that control the equipment. Your use of the machine will then be automatically logged and you will be charged according to the fee schedule below.
C. The facility is available for use 7 X 24. After normal working hours ( 9 -5 Mon –Fri ) lock the door when you leave, and access using a key obtained from the facility.
D. Training is required before using all machines. This can be done by individually with the facility manager, or by experienced users in the various gene center laboratories. For the two confocals you must be trained by the facility managers. Once you have complete the traing course your account will be activated for the confocal.
E. If you encounter problems with the facility E-mail the facility director Lloyd Williams at Williams@genectr.hunter.cuny.edu, or his assistant Zhong Wang at ZWang@genectr.hunter.cuny.edu
F. Do not wear latex gloves in the facility
G. Do not leave your samples in the facility
H. Turn off all microscope lamps after use. It is particularly important to turn off the mercury lamps. Once mercury lamps have been turned off do not turn them back on until they have cooled down for 15 mins
I. Report mercury lamps in service for more than 300 hours
J. Equipment is available on a first come first serve basis. However you can book 2 hours slots on the microscopes using the calendars on the door of the microscope room 826 B. If you come 30 minutes late you will loose your reservation
K. Clean oil off the microscope objective lenses after use.
L. Report all accidents (injuries, spills, fires) to the Security (x4444) and Health and Safety Officer, Ricarod Franco (x4462)
M You must log in to use the equipment using your own Gene Center account.
N Publications using data taken in this facility must acknowlege the RCMI program and the NCRR. |
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Fee Schedule
PerkinElmer Spinning Disk Microscope: The facility charges $20 per hour for use of this microscope. There is a $20 minimum charge, and fractions of an hour count as whole hours. For long time duration experiment, we have a special rate policy described as follows: in any 24 hour period
0 – 4 hours $20/hour
4 – 10 hours $10/hour
10 – 24 hours $5/hour
Email Zhong Wang ( zwang@genectr.hunter.cuny.edu in advance for applying this policy.
Leica Sp2 Confocal: The facility charges $15 per hour for use of the confocal. There is a $15 minimum charge, and fractions of an hour count as whole hours.
Nikon Upright & Inverted Microscopes: The facility charges $5 per hour for use of the microscope. There is a $5 minimum charge, and fractions of an hour count as whole hours. Please sign the log books.
Gemini Spectrophotometer, Typhoon 9410 Imager: The facility charges $5 per scan. Use is monitored by the event log on the computer attached to the machines.
Densitometer, PowerWave HT Plate Reader: The facility charges $3 per scan. Use is monitored by the event log on the computer attached to the machines.
Volocity Analysis Workstation: The facility charges $10 per hour for use of this image analysis package. There is a $10 minimum charge, and fractions of an hour count as whole hours.
Printing: Inkjet usage is charged at 15 ˘/per page, color laser prints at 15 ˘/per page, black and white laser prints at 7 ˘/per page. Usage is monitored automatically by Print Manager Plus application. |
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